Research Laboratories

Musculoskeletal Functional Assessment Center: Pediatric Orthopaedic Research Lab

The Musculoskeletal Functional Assessment Center: Pediatric Orthopaedic Research Lab is located in the Pediatric Orthopaedic Clinic at the Children’s Hospital of Wisconsin. There is a 200 sq. ft area adjacent to the orthopaedic clinic and imaging rooms.

Equipment:

Quantec Spinal Image System for measuring spinal curvature: PC based workstation, calibration device, projector, and mechanical device. Recently the Milwaukee Spinal Scanner System for measuring spinal curvature has been developed, including hand held laser scanner, custom spinal curvature measurement software, and patient stabilizing apparatus. EOS 3D X-ray Orthopedic Imaging System that allows low radiation 3D spinal X-rays while the patient is standing.

Personnel:

The laboratory is supported and run by Dr. Xue-Cheng Liu (Lab Director).

Funding:

The laboratory is supported by Children’s Hospital of Wisconsin.


The Orthopaedic Biomechanics Laboratory

The Orthopaedic Biomechanics Lab is located at Marquette University in Cramer Hall, Room 165, (800 ft2), and includes space for specimen storage, dissection and preparation space for laboratory experiments. This lab is designed and maintained to support basic science and applied research projects in orthopaedic biomechanics. Research methods often encompass in-vitro experiments with human or animal cadaveric specimens and the use of computer modeling and analysis.

Equipment:

Available equipment includes: 1) MTS 809 servo hydraulic axial-torsion material testing system with a pair of hydraulic grips, 8 additional analogue data collection channels, and FlexTest 40 controller; 2) Optotrak Certus Motion Analysis System with 8 additional analogue data collection channels; 3) customized load frame for testing with static loads; 4) an equine portable radiograph unit; 5) Tekscan K-Scan joint pressure measurement system with software and five sensors; 6) AMTI six-axis load-cell and signal amplifier and other uni-axial load cells; 7) Microstrain 3mm micro-miniature DVRTs, 8) various LVDTs displacement transducers; 9) miniature pressure transducers, 10) assorted power and manual tools and surgical instruments.

Personnel:

The Laboratory is staffed with a full-time engineer who holds a degree in Electrical Engineering and Computer Science and twenty years of experience of working in the Lab.

Funding:

The laboratory is supported by the general operating funds of the Department of Orthopaedic Surgery and grants.


OREC Biomaterials Research Laboratories

OREC Biomaterials Research Labs and Offices are located on the Marquette University Campus in the Physics Building, Room 127 (1,250 ft2) and offices (250 ft2) contain tissue processing equipment as well as a darkroom (84 ft2).

Equipment:

Available equipment includes: Leica ASP 300 tissue processor, Shandon Hypercenter XP tissue processor, Leica paraffin embedding center, several microtomes, including a Olympus CUT 4055 and a Reichert Jung sledge microtome, A model high-resolution radiography system (Model 43805 Faxitron), histological staining area under a fume hood, several microscopes including a Zeiss Universal (capable of ultraviolet fluorescence, phase contrast, polarized light, and photomicrography), high-resolution Faxitron X-ray unit, Siemens K-2 microradiography unit, six diamond saws (four high speed, two low speed), grinding machine for undecalcified histological sections, and analytical balance. An Image Pro-Plus image analysis system as well as digital cameras can be coupled to any of the microscopes for quantitative histomorphometric measurements of histological sections.

Personnel:

The OREC Biomaterials Research laboratory is directed by Jeffrey Toth, BSE, PhD, FAIMBE. Dr. Toth’s research expertise includes: Bone histology and histomorphometry, Bone Grafts and bone graft substitutes, Fabrication, characterization, and evaluation of biomaterials, Characterization and pre-clinical testing of orthopaedic biomaterials, and Mechanisms and clinical uses for osteoinductive substances and materials. Research laboratory is staffed by Sara Landschoot, HTL. Sara is a registered histotechnologist. She is HTL certified by The American Society of Clinical Pathologists. Sara has experience in histologic techniques, including: routine and special staining; enzyme histochemistry; immunohistochemistry; electron microscopy; molecular pathology; cytogenetics; Mohs; cytology; grossing; and photography.

Funding:

The laboratory is supported by Marquette University and external grants and resources.


Center for Motion Analysis: (CMA) at the Medical College of Wisconsin

Center for Motion Analysis facilities provide a 2,325 sq. ft. test area, examination/preparation area, offices, and storage at the Children’s Hospital of Wisconsin Greenway Clinic. A 30 ft. walkway is included in the test area for collection of ambulatory data. A 1,071 sq. ft. area is located adjacent to the testing area for support personnel and includes an examination room, equipment storage room and test bench, two offices and a working community area for research fellows and students.

Equipment:

Equipment in the laboratory includes twelve T40 Vicon cameras for motion capture, two AMTI 6 D.O.F Force Plates, two Bertec 6 D.O.F Force Plates,1 Novel EMED pressure platform, 1 Novel PEDAR insole pressure measurement system, F-Scan foot insole pressure measurement system, 16 channel Motion Lab Systems EMG system (surface and fine wire), 8 channel Noraxon surface and fine wire EMG system, adult walker instrumented with two-6 D.O.F. force transducers (AMTI), Biodex rehabilitative treadmill and Biodex extremity evaluation system, Vicon Nexus software for data collection and processing, Vicon Polygon software for constructing reports, Vicon Body Builder software for model construction, EMG analysis software for Motion Lab System and Noraxon, FANDACAL – Foot and ankle motion analysis software, Walker Assisted Gait (WAG) torso and upper extremity motion analysis software, and Matlab software.

Personnel:

Educational support through clinical training and research project participation is provided by Dr. Roger Lyon (Medical Director), Dr. Xue-Cheng Liu (Co-Director), Dr. Gerald Harris (Co-Director), Jessica Fritz, PhD (Research Engineer).

Funding:

The laboratory is supported by Children’s Hospital of Wisconsin.


The Cell Biology Laboratory

Space:

The Cell Biology Laboratory is located on the 4th floor in the Medical Education Building and is situated among the Anesthesiology Research Laboratories with access to shared equipment and expertise.

Equipment:

Tissue culture equipment including incubator, hood, liquid nitrogen tank, centrifuges, water baths and refrigerators are available as well as gel electrophoresis equipment and software for quantitation, thermocyclers for reverse transcription and the polymerase chain reaction (RT-PCR), and an ELISA plate reader. Shared equipment includes ultracold refrigerators, ultracentrifuges, fluorescence spectroscopy, UV-visible spectroscopy, confocal microscopy and animal surgical facilities.

Personnel:

Dr. James Ninomiya (Lab Director) and Janine Struve (Research Associate) support residents in the laboratory.

Funding:

The laboratory is supported by the Medical College of Wisconsin.


Sports Medicine Gait and Motion Analysis Laboratory

The Sports Medicine Gait and Motion Analysis Laboratory is 1600 ft2 located within the Froedtert and Medical College Sports Medicine Center.

Equipment:

Equipment in the laboratory includes a Motion Analysis system with 8 Eagle cameras and Silk heavy duty tripods, 2 force plates (AMTI MiniAmp MSA-6), 4 marker sets, one Jugs radar gun and tripod, a desktop CPU and Cortex 3 software and license, a Hub and switch, a laptop with Cortex 3 software and license, a color printer, and accessory display, 1TB external hard drive, 2 pitching mounds, 2 desks, a filing cabinet.

Personnel:

William Raasch, MD (Medical Director) & Janelle Cross, PhD (Research Director)

Funding:

The laboratory is supported by FMLH and general operating funds of the Department of Orthopaedic Surgery.