Biomaterials

Orthopaedic Surgery Biomaterials and Histopathology Laboratory

The Biomaterials Lab has done its most significant work in the study of calcium phosphate materials. In conjunction with the Medical College of Wisconsin’s Animal Research Center and Clement J. Zablocki VA Medical Center, the lab studies new implant materials compatibility. The lab also collaborates with Marquette University’s Biomaterials program.

Residents and students can become involved in implant studies and basic research that will help them evaluate new products throughout their careers.


Space

OREC Biomaterials Research Labs and Offices are located on the Marquette University Campus in the Physics Building, Room 127 (1,250 ft2) and offices (250 ft2) contain tissue processing equipment as well as a darkroom (84 ft2).


Equipment

slicer(2)To evaluate implants and implant materials, the biomaterials and histopathology lab is equipped with embedding stations, a rotary microtome, a Jung microtome and diamond saws, a tissue pathology laboratory, and a darkroom equipped for microradiography and autoradiography. Histomorphology and microdensitometry of bone also are performed.


Specifically available equipment includes

scopeLeica ASP 300 tissue processor, Shandon Hypercenter XP tissue processor, Leica paraffin embedding center, several microtomes, including a Olympus CUT 4055 and a Reichert Jung sledge microtome, A model high-resolution radiography system (Model 43805 Faxitron), histological staining area under a fume hood, several microscopes including a Zeiss Universal (capable of ultraviolet fluorescence, phase contrast, polarized light, and photomicrography), high-resolution Faxitron X-ray unit, Siemens K-2 microradiography unit, six diamond saws (four high speed, two low speed), grinding machine for undecalcified histological sections, and analytical balance. An Image Pro-Plus image analysis system as well as digital cameras can be coupled to any of the microscopes for quantitative histomorphometric measurements of histological sections.


Personnel

tothThe OREC Biomaterials Research Laboratory is directed by Jeffrey Toth, BSE, PhD, FAIMBE. Dr. Toth’s research expertise includes: Bone histology and histomorphometry, Bone Grafts and bone graft substitutes, Fabrication, characterization, and evaluation of biomaterials, Characterization and pre-clinical testing of orthopaedic biomaterials, and Mechanisms and clinical uses for osteoinductive substances and materials.

Research laboratory is staffed by Sara Landschoot, HTL. Sara is a registered histotechnologist. She is HTL certified by The American Society of Clinical Pathologists. Sara has experience in histologic techniques, including: routine and special staining; enzyme histochemistry; immunohistochemistry; electron microscopy; molecular pathology; cytogenetics; Mohs; cytology; grossing; and photography.


Funding

The laboratory is supported by Marquette University and external grants and resources.